MBNL1-RNA Interactions: Binding-Induced Rna Conformational Changes
نویسندگان
چکیده
منابع مشابه
Ligand-induced conformational changes observed in single RNA molecules.
We present the first demonstration that fluorescence resonance energy transfer can be used to track the motion of a single molecule undergoing conformational changes. As a model system, the conformational changes of individual three-helix junction RNA molecules induced by the binding of ribosomal protein S15 or Mg(2+) ions were studied by changes in single-molecule fluorescence. The transition ...
متن کاملRNA Splicing Is Responsive to MBNL1 Dose
Myotonic dystrophy (DM1) is a highly variable, multi-system disorder resulting from the expansion of an untranslated CTG tract in DMPK. In DM1 expanded CUG repeat RNAs form hairpin secondary structures that bind and aberrantly sequester the RNA splice regulator, MBNL1. RNA splice defects resulting as a consequence of MBNL1 depletion have been shown to play a key role in the development of DM1 p...
متن کاملMutation-induced changes in RNA polymerase-lac ps promoter interactions.
A composite rate assay has been applied to investigate the rate and mechanism of formation of open promoter complexes. Seven DNA templates were studied which were related to the lac ps promoter by single base pair changes in the -10 or -35 region of promoter sequence homology. These small changes induce a nearly 3 order of magnitude variation in the rate of open complex formation. This variatio...
متن کاملElectrostatic Interactions in RNA Aminoglycosides Binding
Aminoglycoside antibiotics interact with seemingly unrelated families of RNA molecules. They bind to 16S ribosomal RNA and interfere with the decoding mechanism by distorting the codon-anticodon recognition.1,2 Similar aminoglycosides specifically inhibit self-splicing group I introns3 and the hammerhead ribozyme.4 Recently, these antibiotics have been found to competitively block the binding o...
متن کاملLomofungin and dilomofungin: inhibitors of MBNL1-CUG RNA binding with distinct cellular effects
Myotonic dystrophy type 1 (DM1) is a dominantly inherited neuromuscular disorder resulting from expression of RNA containing an expanded CUG repeat (CUG(exp)). The pathogenic RNA is retained in nuclear foci. Poly-(CUG) binding proteins in the Muscleblind-like (MBNL) family are sequestered in foci, causing misregulated alternative splicing of specific pre-mRNAs. Inhibitors of MBNL1-CUG(exp) bind...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2012
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2011.11.438